Reconstitution and storage
Handling lyophilized peptides so the material in the vial still matches the certificate.
Before opening
Let the sealed vial equilibrate to room temperature. Opening a cold vial draws in moist air, and water is the main enemy of a lyophilized powder.
Adding solvent
Run the solvent slowly down the inside wall of the vial rather than directly onto the cake. Swirl gently until dissolved; vigorous shaking can degrade longer sequences.
After reconstitution
Keep the solution refrigerated, protected from light, and avoid repeated freeze-thaw cycles. Aliquot if the protocol requires multiple withdrawals.